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Figure 1: Exemplary correlation function of a DLS measurement. In this fourth white paper of the series, we address frequently asked questions related to the application of DLS to the characterization of protein therapeutic formulations. Dynamic light scattering (DLS) results DLS measurements provide results as the hydrodynamic diameter (HDD) and peak size (see Figure 1 and 2). The sensitivity of some modern systems is such that it can also now be used to measure the size of. In this four-part white paper series, common issues and questions surrounding the principles, measurements and analysis of DLS data are discussed in order to help minimize the time required for and complexity of acquiring and interpreting DLS data that is critical throughout the development process. Dynamic Light Scattering, sometimes referred to as Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering, is a technique classically used for measuring the size of particles typically in the sub-micron region, dispersed in a liquid. With that said, the challenge that remains with the application of DLS to protein therapeutic formulations is centered around data interpretation. 1 2 DDM is suitable for typical soft materials such as for instance liquids or gels made of colloids, polymers and liquid crystals but also for biological materials like bacteria and cells. Dynamic Light Scattering (), sometimes referred to as Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering, is a technique classically used for measuring the size of particles typically in the sub-micron region, dispersed in a liquid. The popularity of DLS within the biopharmaceutical industry is a consequence of its wide working size and extended sample concentration ranges, as well as its low volume requirements. Differential dynamic microscopy (DDM) is an optical technique that allows performing light scattering experiments by means of a simple optical microscope. FAQsįrequently asked questions related to the application of DLS to the characterization of protein therapeutic formulations.Ī Malvern Instruments' Bioscience Development Initiative Executive Summaryĭynamic light scattering (DLS) is an analytical technique used to measure the particle size distribution of protein formulations across the oligomer and sub-micron size ranges of approximately 1 nm to 1 µm. What is Dynamic Light Scattering Dynamic light scattering refers to measurement and interpretation of light scattering data on a microsecond time scale. DLS is based on the determination of the diffusion coefficient of particles freely moving in a fluid, a task that can be tackled by measuring the auto- or cross. Application of Dynamic Light Scattering to Protein Therapeutic Formulations: Principles, Measurements and Analysis - 4.